{"product_id":"proteintech-20985-1-ap","title":"Proteintech, 20985-1-AP, DEPDC6\/Deptor Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DEPDC6\/Deptor (20985-1-AP) by Proteintech is a Polyclonal antibody targeting DEPDC6\/Deptor in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n20985-1-AP targets DEPDC6\/Deptor in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells,  A2780 cells,  COLO 320 cells,  HEK-293 cells,  MCF-7 cells\nPositive IHC detected in: human liver cancer tissue,  human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K\/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: DEP domain containing 6\nCalculated Molecular Weight: 46 kDa\nObserved Molecular Weight: 40-45 kDa\nGenBank Accession Number: NM_022783\nGene Symbol: DEPDC6\nGene ID (NCBI): 64798\nRRID: AB_11182391\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8TB45\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868940161193,"sku":"20985-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-20985-1-ap","provider":"Iright","version":"1.0","type":"link"}