{"product_id":"proteintech-21091-1-ap","title":"Proteintech, 21091-1-AP, HDDC3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HDDC3 (21091-1-AP) by Proteintech is a Polyclonal antibody targeting HDDC3 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n21091-1-AP targets HDDC3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, HeLa cells,  human heart tissue,  mouse heart tissue,  mouse kidney tissue,  mouse lung tissue,  mouse pancreas tissue,  mouse testis tissue,  A549 cells,  rat heart tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human prostate cancer tissue, human brain tissue,  human breast cancer tissue,  human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMESH1, encodes by HDDC3 in human and Q9VAM9 in Drosophila melanogaster, was identified as functional SpoT orthologs in metazoan (PMID: 20818390). MESH1 is a cytosolic NADPH phosphatase that is induced under stress conditions, leading to the NADPH depletion and ferroptosis-a novel form of iron-dependent regulated cell death characterized by lipid peroxidation. Accordingly, MESH1 removal preserves the NADPH level in stressed cells and promotes their ferroptotic survival(PMID: 32462112, PMID: 34294679).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15342 Product name: Recombinant human HDDC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC033794 Sequence: MGSEAAQLLEAADFAARKHRQQRRKDPEGTPYINHPIGVARILTHEAGITDIVVLQAALLHDTVEDTDTTLDEVELHFGAQVRRLVEEVTDDKTLPKLERKRLQVEQAPHSSPGAKLVKLADKLYNLRDLNRCTPEVKIQ Predict reactive species\nFull Name: HD domain containing 3\nCalculated Molecular Weight: 179 aa, 20 kDa\nObserved Molecular Weight: 22-25 kDa\nGenBank Accession Number: BC033794\nGene Symbol: HDDC3\nGene ID (NCBI): 374659\nRRID: AB_10733121\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8N4P3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869464547497,"sku":"21091-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-21091-1-ap","provider":"Iright","version":"1.0","type":"link"}