{"product_id":"proteintech-21126-1-ap","title":"Proteintech, 21126-1-AP, Claudin 18 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Claudin 18 (21126-1-AP) by Proteintech is a Polyclonal antibody targeting Claudin 18 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n21126-1-AP targets Claudin 18 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse kidney tissue\nPositive IP detected in: mouse stomach tissue\nPositive IHC detected in: human stomach tissue, human stomach cancer tissue,  human ovary tumor tissue,  human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MKN-45 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nClaudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15517 Product name: Recombinant human CLDN18 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 185-261 aa of BC146668 Sequence: TLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV Predict reactive species\nFull Name: claudin 18\nCalculated Molecular Weight: 261 aa, 28 kDa\nObserved Molecular Weight: 28-29 kDa\nGenBank Accession Number: BC146668\nGene Symbol: CLDN18\nGene ID (NCBI): 51208\nRRID: AB_10733638\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P56856\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868932722857,"sku":"21126-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-21126-1-ap","provider":"Iright","version":"1.0","type":"link"}