{"product_id":"proteintech-21214-1-ap","title":"Proteintech, 21214-1-AP, PLOD2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PLOD2 (21214-1-AP) by Proteintech is a Polyclonal antibody targeting PLOD2 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n21214-1-AP targets PLOD2 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Caco-2 cells, A431 cells,  DU 145 cells,  HT-1080 cells,  THLE-2 cells,  HEK-293 cells,  HeLa cells,  HepG2 cells\nPositive IP detected in: mouse testis tissue\nPositive IHC detected in: human liver cancer tissue, human pancreas tissue,  human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rabbit, zebrafish\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15614 Product name: Recombinant human PLOD2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 459-540 aa of BC037169 Sequence: PYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTA Predict reactive species\nFull Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2\nCalculated Molecular Weight: 758 aa, 85 kDa\nObserved Molecular Weight: 87 kDa\nGenBank Accession Number: BC037169\nGene Symbol: PLOD2\nGene ID (NCBI): 5352\nRRID: AB_10733347\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O00469\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868841824425,"sku":"21214-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-21214-1-ap","provider":"Iright","version":"1.0","type":"link"}