{"product_id":"proteintech-21312-1-ap","title":"Proteintech, 21312-1-AP, DEPDC6 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DEPDC6 (21312-1-AP) by Proteintech is a Polyclonal antibody targeting DEPDC6 in WB, IHC, ELISA applications with reactivity to human samples\n21312-1-AP targets DEPDC6 in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells,  COLO 320 cells,  HT-1080 cells\nPositive IHC detected in: human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15959 Product name: Recombinant human DEPDC6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 60-409 aa of BC024746 Sequence: YPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSSKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKIVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVSNLILTGPRTIVMEVMEELEC Predict reactive species\nFull Name: DEP domain containing 6\nCalculated Molecular Weight: 409 aa, 46 kDa\nObserved Molecular Weight: 55 kDa\nGenBank Accession Number: BC024746\nGene Symbol: DEPDC6\nGene ID (NCBI): 64798\nRRID: AB_2878837\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8TB45\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887162708137,"sku":"21312-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-21312-1-ap","provider":"Iright","version":"1.0","type":"link"}