{"product_id":"proteintech-21570-1-ap","title":"Proteintech, 21570-1-AP, ATP6V0A4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ATP6V0A4 (21570-1-AP) by Proteintech is a Polyclonal antibody targeting ATP6V0A4 in WB, IHC, ELISA applications with reactivity to human, mouse samples\n21570-1-AP targets ATP6V0A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse kidney tissue\nPositive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness. This antibody was generated against the internal region of human ATP6V0A4 and is predicted to detect the a4 isoform only.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag16095 Product name: Recombinant human ATP6V0A4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 190-375 aa of BC109305 Sequence: ERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPERAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAG Predict reactive species\nFull Name: ATPase, H+ transporting, lysosomal V0 subunit a4\nCalculated Molecular Weight: 840 aa, 96 kDa\nObserved Molecular Weight: 100 kDa\nGenBank Accession Number: BC109305\nGene Symbol: ATP6V0A4\nGene ID (NCBI): 50617\nRRID: AB_2918072\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9HBG4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869445902505,"sku":"21570-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-21570-1-ap","provider":"Iright","version":"1.0","type":"link"}