{"product_id":"proteintech-21832-1-ap","title":"Proteintech, 21832-1-AP, ZP2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ZP2 (21832-1-AP) by Proteintech is a Polyclonal antibody targeting ZP2 in WB, IF-P, ELISA applications with reactivity to human, mouse, rat samples\n21832-1-AP targets ZP2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SKOV-3 cells, mouse brain tissue,  mouse ovary tissue,  rat brain tissue\nPositive IF-P detected in: mouse ovary tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nZP2 is a major component of the zona pellucida to which sperm bind prior to penetrating the zona matrix and fusing with the egg. Following fertilization and cortical granule exocytosis, ZP2 is cleaved by ovastacin after which sperm do not bind to the zona pellucida. The zona pellucida and ovastacin proteins transverse the endomembrane system during oogenesis during which passage ZP2 remains intact. Secreted ZP2 is incorporated into the extracellular zona pellucida surrounding ovulated eggs and is cleaved in the zona matrix surrounding 2-cell embryos. (PMID: 28114310)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag16386 Product name: Recombinant human ZP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 440-745 aa of BC096304 Sequence: EIHALWTDFPPSKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWNVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH Predict reactive species\nFull Name: zona pellucida glycoprotein 2 (sperm receptor)\nCalculated Molecular Weight: 745 aa, 82 kDa\nObserved Molecular Weight: 75-82 kDa\nGenBank Accession Number: BC096304\nGene Symbol: ZP2\nGene ID (NCBI): 7783\nRRID: AB_3669375\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q05996\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887954251945,"sku":"21832-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-21832-1-ap","provider":"Iright","version":"1.0","type":"link"}