{"product_id":"proteintech-21913-1-ap","title":"Proteintech, 21913-1-AP, TMEM65 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TMEM65 (21913-1-AP) by Proteintech is a Polyclonal antibody targeting TMEM65 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n21913-1-AP targets TMEM65 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  Neuro-2a cells,  mouse brain tissue,  rat brain tissue\nPositive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Huh-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTMEM65 (Transmembrane protein 65) is an inner-mitochondrial-membrane protein essential for calcium homeostasis and organelle integrity.  By binding to and stabilizing the Na⁺\/Ca²⁺ exchanger NCLX, it facilitates mitochondrial Ca²⁺ efflux, thereby regulating oxidative phosphorylation and limiting ROS production. In cardiomyocytes, TMEM65 localizes to intercalated discs where it interacts with connexin-43, ensuring normal electrical conduction and preventing arrhythmia. Loss-of-function mutations cause mitochondrial myopathy with neurodevelopmental delay, whereas its over-expression in gastric cancer activates the YWHAZ-PI3K-Akt-mTOR axis, promoting tumorigenesis. Thus, TMEM65 links mitochondrial quality control to cytoplasmic signaling, positioning it as a potential therapeutic target in metabolic and oncologic diseases.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15509 Product name: Recombinant human TMEM65 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-178 aa of BC032396 Sequence: MEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPGQLRYVFIHNAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVGVTIGCILGMFPLIFFGGGEEDEKLETKS Predict reactive species\nFull Name: transmembrane protein 65\nCalculated Molecular Weight: 240 aa, 26 kDa\nObserved Molecular Weight: 20-25 kDa\nGenBank Accession Number: BC032396\nGene Symbol: TMEM65\nGene ID (NCBI): 157378\nRRID: AB_2918075\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q6PI78\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887833338025,"sku":"21913-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-21913-1-ap","provider":"Iright","version":"1.0","type":"link"}