{"product_id":"proteintech-22020-1-ap","title":"Proteintech, 22020-1-AP, TERF2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TERF2 (22020-1-AP) by Proteintech is a Polyclonal antibody targeting TERF2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n22020-1-AP targets TERF2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, MCF-7 cells,  NIH\/3T3 cells,  Jurkat cells,  K-562 cells\nPositive IP detected in: K-562 cells\nPositive IHC detected in: human gliomas tissue, rat brain tissue,  mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTRF2, also named as TRBF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TRF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TRF2 together with DCLRE1B\/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TRF2 recruits TERF2IP\/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag16798 Product name: Recombinant human TERF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-251 aa of BC024890 Sequence: MAGGGGSSDGSGRAAGRRASRSSGRARRGRHEPGLGGPAERGAGEARLEEAVNRWVLKFYFHEALRAFRGSRYGDFRQIRDIMQALLVRPLGKEHTVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTEAVVESSRKLVKEAAVIICIKNKEFEKASKILKKHMSKDPTTQKLRNDLLNIIREKNLAHPVIQNFSYETFQQKMLRFLESHLDDAEPYLLTVRLGPSPITMVCP Predict reactive species\nFull Name: telomeric repeat binding factor 2\nCalculated Molecular Weight: 56 kDa\nObserved Molecular Weight: 65-69 kDa\nGenBank Accession Number: BC024890\nGene Symbol: TERF2\nGene ID (NCBI): 7014\nRRID: AB_10973675\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q15554\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869503639721,"sku":"22020-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-22020-1-ap","provider":"Iright","version":"1.0","type":"link"}