{"product_id":"proteintech-22268-1-ap","title":"Proteintech, 22268-1-AP, VAMP7\/TI-VAMP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe VAMP7\/TI-VAMP (22268-1-AP) by Proteintech is a Polyclonal antibody targeting VAMP7\/TI-VAMP in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n22268-1-AP targets VAMP7\/TI-VAMP in WB, IHC, IF\/ICC, IP, ELISA, Blocking applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, rat brain tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human liver tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nVAMP7, also named SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and\/or fusion of transport vesicles to their target membrane during the transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is necessary for calcium-regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: vesicle-associated membrane protein 7\nCalculated Molecular Weight: 25 kDa\nObserved Molecular Weight: 20-25 kDa\nGenBank Accession Number: NM_005638\nGene Symbol: VAMP7\nGene ID (NCBI): 6845\nRRID: AB_11182718\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P51809\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868961886377,"sku":"22268-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-22268-1-ap","provider":"Iright","version":"1.0","type":"link"}