{"product_id":"proteintech-22503-1-ap","title":"Proteintech, 22503-1-AP, CNOT9 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CNOT9 (22503-1-AP) by Proteintech is a Polyclonal antibody targeting CNOT9 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n22503-1-AP targets CNOT9 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, MCF-7 cells,  mouse thymus tissue,  HepG2 cells\nPositive IP detected in: MCF-7 cells\nPositive IHC detected in: human stomach cancer tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, hTERT-RPE1 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag18197 Product name: Recombinant human RQCD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 28-269 aa of BC007102 Sequence: MHSLATAAPVPTTLAQVDREKIYQWINELSSPETRENALLELSKKRESVPDLAPMLWHSFGTIAALLQEIVNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSLGVTGALVKTDEQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVAMILGKMVLQLSKEPSARLLKHVVRCYLRLSDN Predict reactive species\nFull Name: RCD1 required for cell differentiation1 homolog (S. pombe)\nCalculated Molecular Weight: 299 aa, 34 kDa\nObserved Molecular Weight: 30-40 kDa\nGenBank Accession Number: BC007102\nGene Symbol: CNOT9\nGene ID (NCBI): 9125\nRRID: AB_11232413\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q92600\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868876001449,"sku":"22503-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-22503-1-ap","provider":"Iright","version":"1.0","type":"link"}