{"product_id":"proteintech-24289-1-ap","title":"Proteintech, 24289-1-AP, RFTN1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RFTN1 (24289-1-AP) by Proteintech is a Polyclonal antibody targeting RFTN1 in WB, IHC, ELISA applications with reactivity to human samples\n24289-1-AP targets RFTN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells,  Raji cells\nPositive IHC detected in: human tonsillitis tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRaftlin (RFTN1) was originally identified as a major raft protein with molecular mass of 63 kDa in B cells that colocalized with BCR in the lipid raft before and after BCR activation. RFTN1 possesses fatty acylation sites at the N terminus, it predominantly localizes in the cytoplasm in human epithelial cells and monocyte-derived DCs (Mo-DCs) (PMID: 27022195). Human RFTN1 protein estimated to be 63~70 kDa bySDS-PAGE, and this discrepancy may be due to the acidic nature of the protein, which has a calculated isoelectric point of 5.3 (PMID: 12805216).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag17264 Product name: Recombinant human RFTN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 29-374 aa of BC100667 Sequence: ETKIDVSYEYRFLEFTTLSAAELPGSSAVRLASLRDLPAQLLELYQQGFSLAALHPFVQPTHEREKTPLEHIFRAILIKKTDRSQKTDLHNEGYILELDCCSSLDHPTDQKLIPEFIKKIQEAASQGLKFVGVIPQYHSSVNSAGSSAPVSTANSTEDARDAKNARGDHASLENEKPGTGDVCSAPAGRNQSPEPSSGPRGEVPLAKQPSSPSGEGDGGELSPQGVSKTLDGPESNPLEVHEEPLSGKMEIFTLFNKPKSHQKCRQYYPVTIPLHVSKNGQTVSGLDANWLEHMSDHFRKGGMLVNAVFYLGIVNDSLHGLTDGVFIFEAVSTEDSKTIQGYDAIV Predict reactive species\nFull Name: raftlin, lipid raft linker 1\nCalculated Molecular Weight: 578 aa, 63 kDa\nObserved Molecular Weight: 63-70 kDa\nGenBank Accession Number: BC100667\nGene Symbol: RFTN1\nGene ID (NCBI): 23180\nRRID: AB_2879474\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q14699\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869493711017,"sku":"24289-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-24289-1-ap","provider":"Iright","version":"1.0","type":"link"}