{"product_id":"proteintech-24479-1-ap","title":"Proteintech, 24479-1-AP, GID8 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nGID8 Polyclonal Antibody for WB, IHC, IF-P, ELISA\n24479-1-AP targets GID8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, K-562 cells,  HeLa cells,  Jurkat cells,  mouse brain tissue,  rat brain tissue,  SW480 cells,  HepG2 cells\nPositive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse colon tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)-P: IF-P : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe GID8 protein is a component of the Glucose-Induced Degradation-deficient (GID) complex, which plays a key role in ubiquitin-mediated protein degradation, a pathway used to regulate protein turnover in eukaryotic cells. Initially discovered in yeast, GID8 acts as a scaffold protein that is essential for the assembly of the multi-subunit GID complex and for substrate recognition. This complex plays a significant role in metabolic regulation, such as degrading fructose-1,6-bisphosphatase (FBPase) during the shift from gluconeogenesis to glycolysis. In humans, GID8 is part of the CTLH complex, which is associated with various cellular processes including cell cycle regulation, apoptosis, and cancer progression.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag19751 Product name: Recombinant human C20orf11 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-228 aa of BC032120 Sequence: MSYAEKPDEITKDEWMEKLNNLHVQRADMNRLIMNYLVTEGFKEAAEKFRMESGIEPSLDLETLDERIKIREMILKGQIQEAIALINSLHPELLDTNRYLYFHLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECLTEMERTLALLAFDSPEESPFGDLLHTMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKKVKYPKMTDLSKGVIEEPK Predict reactive species\nFull Name: chromosome 20 open reading frame 11\nCalculated Molecular Weight: 228 aa, 27 kDa\nObserved Molecular Weight: 30 kDa\nGenBank Accession Number: BC032120\nGene Symbol: GID8\nGene ID (NCBI): 54994\nRRID: AB_2879562\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NWU2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868955201705,"sku":"24479-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-24479-1-ap","provider":"Iright","version":"1.0","type":"link"}