{"product_id":"proteintech-24500-1-ap","title":"Proteintech, 24500-1-AP, SALL4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SALL4 (24500-1-AP) by Proteintech is a Polyclonal antibody targeting SALL4 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n24500-1-AP targets SALL4 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: mouse testis tissue, human ovary tumor tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Caco-2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSal-like protein 4 (SALL4), also named Zinc finger protein 797, contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is a transcription factor that exists in two splice isoforms and regulates transcription in embryonic stem cells, hematopoiesis, and acute myeloid leukemia. SALL4 is activated in fetal liver tissue, silenced in adult liver tissue, and reexpressed as an oncofetal protein in hepatocellular carcinoma (PMID: 26892498, 23758232). SALL4 has two isoforms with predicted molecular weights of 112 kDa (SALL4A) and  66 kDa (unmodified SALL4B), and SALL4B was found to be the dominant endogenous isoform in HCC cell lines. Ectopically expressed SALL4B migrated at 95 kDa modified by ubiquitination, 130 kDa modified by sumoylation (PMID: 23012367).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag17480 Product name: Recombinant human SALL4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC111714 Sequence: MSRRKQAKPQHINSEEDQGEQQPQQQTPEFADAAPAAPAAGELGAPVNHPGNDEVASEDEATVKRLRREETHVCEKCCAEFFSISEFLEHKKNCTKNPPVLIMNDSEGPVPSEDFSGAVLSHQPTSPGSKDCHRENGGSSEDMKEKPDAESVVYLKTETALPPTPQDISYLAKGKVANTNVTLQALRGTKVAVNQRSADALPAPVPGANSIPWVLEQILCLQQQQLQQIQLTEQIRIQVNMWASHALHSSGAGADTLKTLGSHMSQQVSAAVALLSQKAGSQGLSLDALKQAKLPHANIPSATSSLSPGLAPFTLKPDGTRVLPNVMSRLPSALLPQAPGSVLFQSPFST Predict reactive species\nFull Name: sal-like 4 (Drosophila)\nCalculated Molecular Weight: 1053 aa, 112 kDa\nObserved Molecular Weight: 66-75 kDa\nGenBank Accession Number: BC111714\nGene Symbol: SALL4\nGene ID (NCBI): 57167\nRRID: AB_2879576\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UJQ4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868920893609,"sku":"24500-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-24500-1-ap","provider":"Iright","version":"1.0","type":"link"}