{"product_id":"proteintech-25133-1-ap","title":"Proteintech, 25133-1-AP, HAP1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HAP1 (25133-1-AP) by Proteintech is a Polyclonal antibody targeting HAP1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human,  mouse samples\n25133-1-AP targets HAP1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human,  mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: fetal human brain tissue\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHAP1 (Huntingtin-associated protein 1) was originally identified as neuronal protein that specifically associates with HTT\/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT. HTT is expressed ubiquitously, while HAP1 is expressed predominantly in the central nervous system (CNS). Both HTT and HAP1 participate in intracellular trafficking. HAP1 is involved in the vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation (PMID: 19262167).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag17705 Product name: Recombinant human HAP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 331-671 aa of BC156728 Sequence: LREEASQLDTLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQRCRMYGAETEKLQKQLASEKEIQMQLQEESVWVGSQLQDLREKYMDCGGMLIEMQEEVKTLRQQPPVSTGSATHYPYSVPLETLPGFQETLAEELRTSLRRMISDPVYFMERNYEMPRGDTSSLRYDFRYSEDREQVRGFEAEEGLMLAADIMRGEDFTPAEEFVPQEELGAAKKVPAEEGVMEEAELVSEETEGWEEVELELDEATRMNVVTSALEASGLGPSHLDMNYVLQQLANWQDAHYRRQLRWKMLQKGECPHGALPAASRTSCRSSCR Predict reactive species\nFull Name: huntingtin-associated protein 1\nCalculated Molecular Weight: 671 aa, 76 kDa\nObserved Molecular Weight: 75-80 kDa\nGenBank Accession Number: BC156728\nGene Symbol: HAP1\nGene ID (NCBI): 9001\nRRID: AB_2879915\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P54257\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869410119849,"sku":"25133-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-25133-1-ap","provider":"Iright","version":"1.0","type":"link"}