{"product_id":"proteintech-25417-1-ap","title":"Proteintech, 25417-1-AP, SRPK2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SRPK2 (25417-1-AP) by Proteintech is a Polyclonal antibody targeting SRPK2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples\n25417-1-AP targets SRPK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: mouse testis tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSRPK2, also named as serine\/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine\/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag17885 Product name: Recombinant human SRPK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 293-513 aa of BC035214 Sequence: LEREAERKIIEENITSAAPSNDQDGEYCPEVKLKTTGLEEAAEAETAKDNGEAEDQEEKEDAEKENIEKDEDDVDQELANIDPTWIESPKTNGHIENGPFSLEQQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLV Predict reactive species\nFull Name: SFRS protein kinase 2\nCalculated Molecular Weight: 688 aa, 78 kDa\nObserved Molecular Weight: 105-110 kDa\nGenBank Accession Number: BC035214\nGene Symbol: SRPK2\nGene ID (NCBI): 6733\nRRID: AB_2880068\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P78362\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869771485353,"sku":"25417-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-25417-1-ap","provider":"Iright","version":"1.0","type":"link"}