{"product_id":"proteintech-25625-1-ap","title":"Proteintech, 25625-1-AP, CHCHD3\/MIC19 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CHCHD3\/MIC19 (25625-1-AP) by Proteintech is a Polyclonal antibody targeting CHCHD3\/MIC19 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n25625-1-AP targets CHCHD3\/MIC19 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, human placenta tissue,  HeLa cells,  Jurkat cells,  NIH\/3T3 cells,  NIH\/3T3 cells.,  mouse liver tissue,  rat liver tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:4000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag22579 Product name: Recombinant human CHCHD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC014839 Sequence: MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG Predict reactive species\nFull Name: coiled-coil-helix-coiled-coil-helix domain containing 3\nCalculated Molecular Weight: 227 aa, 26 kDa\nObserved Molecular Weight: 26 kDa\nGenBank Accession Number: BC014839\nGene Symbol: CHCHD3\nGene ID (NCBI): 54927\nRRID: AB_2687533\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NX63\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868853063849,"sku":"25625-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-25625-1-ap","provider":"Iright","version":"1.0","type":"link"}