{"product_id":"proteintech-25862-1-ap","title":"Proteintech, 25862-1-AP, PHF23 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PHF23 (25862-1-AP) by Proteintech is a Polyclonal antibody targeting PHF23 in WB, IF\/ICC, ELISA applications with reactivity to human samples\n25862-1-AP targets PHF23 in WB, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  MCF-7 cells\nPositive IF\/ICC detected in: A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPHD finger protein 23 (PHF23), also known as hJUNE-1b, belongs to the PHF23 family. As a putative epigenetic reader, the PHD domain of PHF23 specifically binds to tri-methylated lysine 4 of histone H3 (H3K4me3). PHF23 is widely expressed in human tissues and various cell lines and exerts a negative effect on autophagy by promoting the degradation of E3 ligase LRSAM1 (leucine-rich repeat and sterile alpha motif containing 1) (PMID: 32978226, 32380078). The observed molecular weight of PHF23 is ~65 kDa, which may be caused by post-translational modification. The specificity of this antibody was demonstrated by siRNA-mediated knockdown of target protein.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag23044 Product name: Recombinant human PHF23 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-403 aa of BC002509 Sequence: MLEAMAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEESDWPASGSSSPLRGESAADSDGWDSAPSDLRTIQTFVKKAKSSKRRAAQAGPTQPGPPRSTFSRLQAPDSATLLEKMKLKDSLFDLDGPKVASPLSPTSLTHTSRPPAALTPVPLSQGDLSHPPRKKDRKNRKLGPGAGAGFGVLRRPRPTPGDGEKRSRIKKSKKRKLKKAERGDRLPPPGPPQAPPSDTDSEEEEEEEEEEEEEEMATVVGGEAPVPVLPTPPEAPRPPATVHPEGVPPADSESKEVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITCYCRKPFAGRPMIECSLCGTWIHLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGPPKSGEP Predict reactive species\nFull Name: PHD finger protein 23\nCalculated Molecular Weight: 403 aa, 44 kDa\nObserved Molecular Weight: ~65 kDa\nGenBank Accession Number: BC002509\nGene Symbol: PHF23\nGene ID (NCBI): 79142\nRRID: AB_3085824\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BUL5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869728133289,"sku":"25862-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-25862-1-ap","provider":"Iright","version":"1.0","type":"link"}