{"product_id":"proteintech-27274-1-ap","title":"Proteintech, 27274-1-AP, TRIM55 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TRIM55 (27274-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM55 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n27274-1-AP targets TRIM55 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: C2C12 cells\nPositive IHC detected in: human liver cancer tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTripartite motif-containing 55 (TRIM55), which is also called muscle-specific ring finger 2 (Murf2), has been indicated to participate in the natural development of muscle and the cardiovascular system. The expression of TRIM55 also inhibited several markers of cancer stem-like cells (PMID: 34974792). MURF2 mRNA studies revealed up to four potential isoforms generated by differential splicing events within the 3′-region of the gene: p60, p50, p27, and p60B MURF2 isoforms. The change in the ratio of E3 ligase isoforms regulates the sequential activation of proteasomal or autophagic degradation of target components(PMID: 15199100, PMID: 24124537). This antibody could detect multiple isoforms or post-translational modifications of TRIM55.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag25338 Product name: Recombinant human TRIM55 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 265-452 aa of BC007750 Sequence: GIQFMDEPEMAVFLQNAKTLLKKISEASKAFQMEKIEHGYENMNHFTVNLNREEKIIREIDFYREDEDEEEEEGGEGEKEGEGEVGGEAVEVEEVENVQTEFPGEDENPEKASELSQVELQAAPGALPVSSPEPPPALPPAADAPVTQIGFEAPPLQGQAAAPASGSGADSEPARHIFSFSWLNSLNE Predict reactive species\nFull Name: tripartite motif-containing 55\nCalculated Molecular Weight: 548 aa, 60 kDa\nObserved Molecular Weight: 60-65 kDa，75-80 kDa\nGenBank Accession Number: BC007750\nGene Symbol: TRIM55\nGene ID (NCBI): 84675\nRRID: AB_3669590\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BYV6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887838843049,"sku":"27274-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-27274-1-ap","provider":"Iright","version":"1.0","type":"link"}