{"product_id":"proteintech-27279-1-ap","title":"Proteintech, 27279-1-AP, KCTD10 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe KCTD10 (27279-1-AP) by Proteintech is a Polyclonal antibody targeting KCTD10 in WB, IHC, IP, ELISA applications with reactivity to human samples\n27279-1-AP targets KCTD10 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  HuH-7 cells,  Jurkat cells,  K-562 cells,  MCF-7 cells,  U2OS cells\nPositive IP detected in: HuH-7 cells\nPositive IHC detected in: human breast cancer tissue, human intrahepatic cholangiocarcinoma tissue,  human prostate hyperplasia tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nKCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag25971 Product name: Recombinant human KCTD10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 253-313 aa of BC040062 Sequence: TLNILLYEAQDGRGPDNALLEATGGAAGRSHHLDEDEERERIERVRRIHIKRPDDRAHLHQ Predict reactive species\nFull Name: potassium channel tetramerisation domain containing 10\nCalculated Molecular Weight: 313 aa, 35 kDa\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: BC040062\nGene Symbol: KCTD10\nGene ID (NCBI): 83892\nRRID: AB_2880827\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9H3F6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869413855401,"sku":"27279-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-27279-1-ap","provider":"Iright","version":"1.0","type":"link"}