{"product_id":"proteintech-27463-1-ap","title":"Proteintech, 27463-1-AP, TRIM8 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TRIM8 (27463-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM8 in WB, IHC, ELISA applications with reactivity to human samples\n27463-1-AP targets TRIM8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, L02 cells\nPositive IHC detected in: human breast cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTRIM8 (Tripartite motif containing protein 8), also known as RNF27 (RING finger protein 27), a member of TRIM family proteins, is known to play a dual role as both tumor suppressor and oncogene, and to function at the crosstalk of cancer and innate immunity. TRIM8 can function at two subcellular sites-nucleus and cytoplasm-to regulate NF-κB, one of the central signaling pathways that plays a critical role in carcinogenesis and inflammatory diseases (PMID: 33230447, 22262183). TRIM8 is composed of 551 amino acids (aa) with a molecular weight of 61.5 kDa, contains a Nuclear Localization Signal (NLS), and forms specific nuclear structures similar to the TRIM19\/PML Nuclear Bodies (NBs) (PMID: 33807506, 29182544).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag26517 Product name: Recombinant human TRIM8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 180-351 aa of BC021925 Sequence: CDVEIRRNEIRKMLMKQQDRLEEREQDIEDQLYKLESDKRLVEEKVNQLKEEVRLQYEKLHQLLDEDLRQTVEVLDKAQAKFCSENAAQALHLGERMQEAKKLLGSLQLLFDKTEDVSFMKNTKSVKILMDRTQTCTSSSLSPTKIGHLNSKLFLNEVAKKEKQLRKMLEGP Predict reactive species\nFull Name: tripartite motif-containing 8\nCalculated Molecular Weight: 61 kDa\nObserved Molecular Weight: 61 kDa\nGenBank Accession Number: BC021925\nGene Symbol: TRIM8\nGene ID (NCBI): 81603\nRRID: AB_2880877\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BZR9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868975157417,"sku":"27463-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-27463-1-ap","provider":"Iright","version":"1.0","type":"link"}