{"product_id":"proteintech-28328-1-ap","title":"Proteintech, 28328-1-AP, UBE2D1\/2\/3\/4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe UBE2D1\/2\/3\/4 (28328-1-AP) by Proteintech is a Polyclonal antibody targeting UBE2D1\/2\/3\/4 in WB, IHC, ELISA applications with reactivity to Human, Mouse, Rat samples\n28328-1-AP targets UBE2D1\/2\/3\/4 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, MCF-7 cells,  NIH\/3T3 cells,  mouse testis tissue,  mouse kidney tissue,  rat kidney tissue,  PC-12 cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nUbiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1\/2\/3\/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, Mouse, Rat\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag28736 Product name: Recombinant human UBE2D2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-59 aa of BC033349 Sequence: MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTD Predict reactive species\nFull Name: ubiquitin-conjugating enzyme E2D 2 (UBC4\/5 homolog, yeast)\nCalculated Molecular Weight: 17 kDa\nObserved Molecular Weight: 14-17 kDa\nGenBank Accession Number: BC033349\nGene Symbol: UBE2D2\nGene ID (NCBI): 7322\nRRID: AB_2881114\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P62837\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869784297641,"sku":"28328-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-28328-1-ap","provider":"Iright","version":"1.0","type":"link"}