{"product_id":"proteintech-28463-1-ap","title":"Proteintech, 28463-1-AP, F4\/80 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe F4\/80 (28463-1-AP) by Proteintech is a Polyclonal antibody targeting F4\/80 in WB, IHC, IF\/ICC, IF-P, IF-Fro, ELISA applications with reactivity to mouse, rat samples\n28463-1-AP targets F4\/80 in WB, IHC, IF\/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: unboiled J774A.1 cells, unboiled RAW 264.7 cells\nPositive IHC detected in: mouse spleen tissue, rat spleen tissue,  rat liver tissue,  mouse lung tissue,  mouse heart tissue,  mouse brain tissue,  mouse liver tissue,  mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse spleen tissue, mouse liver tissue\nPositive IF-Fro detected in: mouse spleen tissue\nPositive IF\/ICC detected in: RAW 264.7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:4000-1:16000\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMouse F4\/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4\/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4\/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag28252 Product name: Recombinant mouse Adgre1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 443-644 aa of NM_010130 Sequence: MELSKEETSTLGTILLETVESTMLAALLIPSGNASQMIQTEYLDIESKVINEECKENESINLAARGDKMNVGCFIIKESVSTGAPGVAFVSFAHMESVLNERFFEDGQSFRKLRMNSRVVGGTVTGEKKEDFSKPIIYTLQHIQPKQKSERPICVSWNTDVEDGRWTPSGCEIVEASETHTVCSCNRMANLAIIMASGELTME Predict reactive species\nFull Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1\nCalculated Molecular Weight: 102 kDa\nObserved Molecular Weight: 130 kDa, 160 kDa\nGenBank Accession Number: NM_010130\nGene Symbol: F4\/80\nGene ID (NCBI): 13733\nRRID: AB_2881149\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q61549\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868821377193,"sku":"28463-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-28463-1-ap","provider":"Iright","version":"1.0","type":"link"}