{"product_id":"proteintech-28653-1-ap","title":"Proteintech, 28653-1-AP, VPS33B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe VPS33B (28653-1-AP) by Proteintech is a Polyclonal antibody targeting VPS33B in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples\n28653-1-AP targets VPS33B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, L02 cells,  mouse testis tissue,  rat testis tissue\nPositive IHC detected in: mouse kidney tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nVPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP\/unc-18\/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking\/fusion reactions of late endosomes\/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag29886 Product name: Recombinant human VPS33B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 248-508 aa of BC016445 Sequence: EGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRD Predict reactive species\nFull Name: vacuolar protein sorting 33 homolog B (yeast)\nCalculated Molecular Weight: 617 aa, 71 kDa\nObserved Molecular Weight: 65 kDa\nGenBank Accession Number: BC016445\nGene Symbol: VPS33B\nGene ID (NCBI): 26276\nRRID: AB_3086076\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9H267\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887920533673,"sku":"28653-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-28653-1-ap","provider":"Iright","version":"1.0","type":"link"}