{"product_id":"proteintech-28735-1-ap","title":"Proteintech, 28735-1-AP, Phospho-p70(S6K) (Thr389)\/p85(S6K) (Thr412) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-p70(S6K) (Thr389)\/p85(S6K) (Thr412) (28735-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-p70(S6K) (Thr389)\/p85(S6K) (Thr412) in WB, ELISA applications with reactivity to human samples\n28735-1-AP targets Phospho-p70(S6K) (Thr389)\/p85(S6K) (Thr412) in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: IGF-1 treated MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine\/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)\/mammalian target of rapamycin (mTOR) pathway. P70(S6K) plays a crucial role in controlling cell cycle, growth and survival. The PI3K\/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85(S6K) and p70(S6K), which is identical to p85(S6K) but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70(S6K) at Thr389 and p85(S6K) at Thr412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat, pig, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1\nObserved Molecular Weight: 65-85 kDa\nGenBank Accession Number: NM_003161\nGene Symbol: p70 S6K\nGene ID (NCBI): 6198\nRRID: AB_2918197\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P23443\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868840808617,"sku":"28735-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-28735-1-ap","provider":"Iright","version":"1.0","type":"link"}