{"product_id":"proteintech-28807-1-ap","title":"Proteintech, 28807-1-AP, Phospho-Chk1 (Ser317) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-Chk1 (Ser317) (28807-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-Chk1 (Ser317) in WB, ELISA applications with reactivity to Human samples\n28807-1-AP targets Phospho-Chk1 (Ser317) in WB, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: UV treated HEK-293T cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM\/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: CHK1 checkpoint homolog (S. pombe)\nCalculated Molecular Weight: 54 kDa\nObserved Molecular Weight: 55 kDa\nGenBank Accession Number: BC004202\nGene Symbol: Chk1\nGene ID (NCBI): 1111\nRRID: AB_3085336\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O14757\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869728592041,"sku":"28807-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-28807-1-ap","provider":"Iright","version":"1.0","type":"link"}