{"product_id":"proteintech-28961-1-ap","title":"Proteintech, 28961-1-AP, Phospho-P53 (Ser15) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-P53 (Ser15) (28961-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-P53 (Ser15) in WB, IF\/ICC, ELISA applications with reactivity to human, rat samples\n28961-1-AP targets Phospho-P53 (Ser15) in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: etoposide treated HT-29 cells, HSC-T6 cells,  UV treated HSC-T6 cells\nPositive IF\/ICC detected in: etoposide treated HT-29 cells, HT-29 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tumor protein p53\nCalculated Molecular Weight: 44 kDa\nObserved Molecular Weight: 53 kDa\nGenBank Accession Number: BC003596\nGene Symbol: P53\nGene ID (NCBI): 7157\nRRID: AB_2881236\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P04637\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868852932777,"sku":"28961-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-28961-1-ap","provider":"Iright","version":"1.0","type":"link"}