{"product_id":"proteintech-28977-1-ap","title":"Proteintech, 28977-1-AP, Phospho-STAT1 (Ser727) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-STAT1 (Ser727) (28977-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-STAT1 (Ser727) in WB, IF\/ICC, ELISA applications with reactivity to human samples\n28977-1-AP targets Phospho-STAT1 (Ser727) in WB, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: IFN gamma treated HeLa cells\nPositive IF\/ICC detected in: IFN alpha treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSTAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators.\nSTAT1 activating phosphorylation at Ser727 is p38-dependant during\nlipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: signal transducer and activator of transcription 1, 91kDa\nCalculated Molecular Weight: 83 kDa\nObserved Molecular Weight: 84 kDa\nGenBank Accession Number: BC002704\nGene Symbol: STAT1\nGene ID (NCBI): 6772\nRRID: AB_2923585\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P42224\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868891205801,"sku":"28977-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-28977-1-ap","provider":"Iright","version":"1.0","type":"link"}