{"product_id":"proteintech-29012-1-ap","title":"Proteintech, 29012-1-AP, Phospho-CHEK2 (Thr68) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-CHEK2 (Thr68) (29012-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-CHEK2 (Thr68) in WB, ELISA applications with reactivity to Human samples\n29012-1-AP targets Phospho-CHEK2 (Thr68) in WB, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MMS treated PC-3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSerine\/threonine-protein kinase Chk2 (CHEK2) is a serine\/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine\/threoninekinase TTK\/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ\/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: CHK2 checkpoint homolog (S. pombe)\nCalculated Molecular Weight: 61 kDa\nObserved Molecular Weight: 65 kDa\nGenBank Accession Number: BC004207\nGene Symbol: CHEK2\nGene ID (NCBI): 11200\nRRID: AB_2918230\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O96017\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868935704745,"sku":"29012-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-29012-1-ap","provider":"Iright","version":"1.0","type":"link"}