{"product_id":"proteintech-29072-1-ap","title":"Proteintech, 29072-1-AP, Phospho-PRAS40 (Thr246) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-PRAS40 (Thr246) (29072-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PRAS40 (Thr246) in WB, ELISA applications with reactivity to Human samples\n29072-1-AP targets Phospho-PRAS40 (Thr246) in WB, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: IGF-1 treated MCF-7 cells, Calyculin A treated PC-3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB\/Akt), nuclear factor kappa-B(NF-κB), proto-oncogene serine\/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2). PGK1 phosphorylated PRAS40 at Threonine 246, which could be inhibited by blocking the interaction. PRAS40 can be phosphorylated by p-AKT at Thr246 site and promote the signal downstream to the mTOR signaling pathway. (PMID: 35058442, PMID: 32855714)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: AKT1 substrate 1 (proline-rich)\nCalculated Molecular Weight: 27 kDa\nObserved Molecular Weight: 40 kDa\nGenBank Accession Number: BC051844\nGene Symbol: PRAS40\nGene ID (NCBI): 84335\nRRID: AB_2935413\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96B36\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887761117353,"sku":"29072-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-29072-1-ap","provider":"Iright","version":"1.0","type":"link"}