{"product_id":"proteintech-29202-1-ap","title":"Proteintech, 29202-1-AP, Di-Methyl-Histone H3 (Lys36) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Di-Methyl-Histone H3 (Lys36) (29202-1-AP) by Proteintech is a Polyclonal antibody targeting Di-Methyl-Histone H3 (Lys36) in WB, ELISA applications with reactivity to human, mouse, rat samples\n29202-1-AP targets Di-Methyl-Histone H3 (Lys36) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: C2C12 cells, HEK-293T cells,  THP-1 cells,  HeLa cells,  HepG2 cells,  HSC-T6 cells\nPositive ELISA detected in: peptide\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nEnzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:32000-1:128000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHistones, including H1\/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).NSD enzymes exhibit an auto-inhibitory state that is relieved by nucleosome engagement, allowing for H3K36 di-methylation catalysis (PMID:22099308).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: histone cluster 1, H3a\nObserved Molecular Weight: 15-18 kDa\nGenBank Accession Number: BC066245\nGene Symbol: HIST1H3A\nGene ID (NCBI): 8350\nRRID: AB_2918247\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P68431\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869674229929,"sku":"29202-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-29202-1-ap","provider":"Iright","version":"1.0","type":"link"}