{"product_id":"proteintech-29771-1-ap","title":"Proteintech, 29771-1-AP, EBP50\/NHERF-1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EBP50\/NHERF-1 (29771-1-AP) by Proteintech is a Polyclonal antibody targeting EBP50\/NHERF-1 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n29771-1-AP targets EBP50\/NHERF-1 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HCT 116 cells, HepG2 cells,  MCF-7 cells,  MDA-MB-231 cells,  rat kidney tissue,  mouse kidney tissue\nPositive IHC detected in: human placenta tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, A549 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNa+\/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters\/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag30878 Product name: Recombinant human SLC9A3R1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 260-358 aa of NM_004252 Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL Predict reactive species\nFull Name: solute carrier family 9 (sodium\/hydrogen exchanger), member 3 regulator 1\nCalculated Molecular Weight: 39 kDa\nObserved Molecular Weight: 50 kDa\nGenBank Accession Number: NM_004252\nGene Symbol: SLC9A3R1\/EBP50\/NHERF-1\nGene ID (NCBI): 9368\nRRID: AB_2935478\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O14745\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887412531369,"sku":"29771-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-29771-1-ap","provider":"Iright","version":"1.0","type":"link"}