{"product_id":"proteintech-30201-1-ap","title":"Proteintech, 30201-1-AP, FCGR3B\/CD16b Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe FCGR3B\/CD16b (30201-1-AP) by Proteintech is a Polyclonal antibody targeting FCGR3B\/CD16b in WB, IF\/ICC, ELISA applications with reactivity to Human, rat samples\n30201-1-AP targets FCGR3B\/CD16b in WB, IF\/ICC, ELISA applications and shows reactivity with Human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Raji cells, U-937 cells,  rat spleen tissue\nPositive IF\/ICC detected in: A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCD16 is a  low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.  In humans, FcγRIIIa\/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb\/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues  in the mature proteins(PMID: 30361439). FCGR3B\/CD16b can be detected as 26 kDa (PMID: 34108956; 36096917) and 50-75 kDa (PMID: 30655272; 18208407). The antibody is specific for FCGR3B\/CD16b.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Recombinant protein Predict reactive species\nFull Name: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)\nCalculated Molecular Weight: 26 kDa\nObserved Molecular Weight: 55 kDa\nGenBank Accession Number: NM_000570\nGene Symbol: FCGR3B\nGene ID (NCBI): 2215\nRRID: AB_3086262\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O75015\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887637680297,"sku":"30201-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-30201-1-ap","provider":"Iright","version":"1.0","type":"link"}