{"product_id":"proteintech-30412-1-ap","title":"Proteintech, 30412-1-AP, Haptoglobin Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Haptoglobin (30412-1-AP) by Proteintech is a Polyclonal antibody targeting Haptoglobin in WB, IP, ELISA applications with reactivity to human, rat, pig, rabbit samples\n30412-1-AP targets Haptoglobin in WB, IP, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rabbit blood tissue, pig plasma,  rabbit plasma\nPositive IP detected in: human plasma\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHaptoglobin is also named as Zonulin and belongs to the peptidase S1 family. Haptoglobin is an alpha-2-glycoprotein synthesized mainly by hepatocytes consisting of both alpha and beta subunits linked by disulfide bonds forming dimers (PMID: 4018023). Haptoglobin is primarily produced in the liver, and when red cells are lysed in vivo, free hemoglobin binds to circulating haptoglobin; the hemoglobin-haptoglobin complex is then degraded by the reticuloendothelial system (PMID: 24809098). Because haptoglobin levels become depleted in the presence of large amounts of free hemoglobin, decreased haptoglobin is a marker of hemolysis. The human haptoglobin (HP) gene exists in two major allelic forms leading to three major genotypes: Hp1-1, Hp2-1, and Hp-2-2, that separate on alkaline starch gel electrophoresis due to different molecular weights (PMID: 17474882). Their properties vary in binding strength, anti-oxidative ability, and rate of clearance. Binding strength is greatest with Hp1-1, but the Hp-2 genotypes can bind a larger number of hemoglobin alpha-beta dimers. Complexes of Hp2-2 and hemoglobin have higher affinity for the CD163 receptor, the monocyte-macrophage receptor that facilitates its clearance. Neutrophils contained a large amount of highly glycosylated Hp (β-chain 45-65 kDa) synthesized in neutrophil precursors and stored in specific granules and a small amount of Hp (β-chain 39 kDa) endocytosed from plasma and stored in secretory vesicles (PMID: 16543473).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat, pig, rabbit\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Recombinant protein Predict reactive species\nFull Name: haptoglobin\nObserved Molecular Weight: 36-45 kDa\nGenBank Accession Number: BC121125\nGene Symbol: HP\nGene ID (NCBI): 3240\nRRID: AB_3669719\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P00738\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887663435945,"sku":"30412-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-30412-1-ap","provider":"Iright","version":"1.0","type":"link"}