{"product_id":"proteintech-30814-1-ap","title":"Proteintech, 30814-1-AP, Phospho-CD117\/c-Kit (Tyr721) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-CD117\/c-Kit (Tyr721) (30814-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-CD117\/c-Kit (Tyr721) in WB, ELISA applications with reactivity to human samples\n30814-1-AP targets Phospho-CD117\/c-Kit (Tyr721) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Mo7e cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nKit is composed of five N-glycosylated immunoglobulin domains in the N-terminal extracellular portion that bind SCF, a transmembrane domain, and the carboxy-terminal intracellular tyrosine kinase domain. The binding of SCF autophosphorylates Kit on specific tyrosine residues (Tyr), such as Tyr568\/570, Tyr703, Tyr721, and Tyr936. Kit then binds to other cytoplasmic enzymes, such as PI3K and Src kinases, and this complex activates downstream molecules. This activates the Akt-Bad pathway and the Ras-Mek-Erk cascade, which regulate gene expression and cytoskeletal structures, resulting in cell proliferation and survival. (PMID: 28403213)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog\nCalculated Molecular Weight: 976 aa, 110 kDa\nObserved Molecular Weight: 145 kDa\nGenBank Accession Number: BC071593\nGene Symbol: CD117\nGene ID (NCBI): 3815\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P10721\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887759413417,"sku":"30814-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-30814-1-ap","provider":"Iright","version":"1.0","type":"link"}