{"product_id":"proteintech-31313-1-ap","title":"Proteintech, 31313-1-AP, Pan-cadherin Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Pan-cadherin (31313-1-AP) by Proteintech is a Polyclonal antibody targeting Pan-cadherin in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n31313-1-AP targets Pan-cadherin in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, C6 cells,  HUVEC cells,  HeLa cells,  MCF-7 cells,  mouse brain tissue,  mouse heart tissue,  human placenta tissue\nPositive IHC detected in: mouse colon tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in tissue morphogenesis and the maintenance of normal tissue architecture (PMID: 23885883). The classic cadherin family members include E-, N-, P-, R-, and B-cadherins. The classic cadherins have ectodomains composed of five extracellular cadherin (EC) repeats, a single transmembrane region, and a cytoplasmic domain. Structural characterization indicated that the known cadherins are glycoproteins ranging in their apparent molecular weights between 120 and 140 kDa (PMID: 2077036). This polyclonal antibody is raised against a synthetic peptide corresponding to a conserved region of human E-cadherin and can detect E-, N-, P-, VE-, and R-cadherins.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: cadherin 1, type 1, E-cadherin (epithelial)\nCalculated Molecular Weight: 97 kDa\nObserved Molecular Weight: 120-130 kDa\nGenBank Accession Number: NM_004360\nGene Symbol: E-cadherin\nGene ID (NCBI): 999\nRRID: AB_3669943\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity Purification\nUNIPROT ID: P12830\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869570060457,"sku":"31313-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-31313-1-ap","provider":"Iright","version":"1.0","type":"link"}