{"product_id":"proteintech-31463-1-ap","title":"Proteintech, 31463-1-AP, HSD17B12 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HSD17B12 (31463-1-AP) by Proteintech is a Polyclonal antibody targeting HSD17B12 in WB, IHC, ELISA applications with reactivity to human samples\n31463-1-AP targets HSD17B12 in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-29 cells, HuH-7 cells,  MDA-MB-231 cells\nPositive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:300-1:1200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHSD17B12 (17β-hydroxysteroid dehydrogenase 12) is an important protein belonging to the hydroxysteroid dehydrogenase family, which is mainly involved in the metabolism of steroids and fatty acids in the body. It has diverse functions in cells and is involved in various biochemical reactions, especially playing a key role in sex hormone synthesis and fatty acid elongation. HSD17B12 can convert estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. HSD17B12 is expressed in a variety of tissues, especially in the brain tissue and the digestive system. In disease states, such as colorectal cancer (CRC), the expression level of HSD17B12 is significantly increased, suggesting that it may play a role in the occurrence and development of tumors.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag34833 Product name: Recombinant human HSD17B12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 34-312 aa of BC012043 Sequence: LRVWGVGNEAGVGPGLGEWAVVTGSTDGIGKSYAEELAKHGMKVVLISRSKDKLDQVSSEIKEKFKVETRTIAVDFASEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMLPVPLLTIYSATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNGYLIHALMGSIISNLPSWIYLKIVMNMNKSTRAHYLKKTKKN Predict reactive species\nFull Name: hydroxysteroid (17-beta) dehydrogenase 12\nCalculated Molecular Weight: 312 aa, 34 kDa\nObserved Molecular Weight: ~34 kDa\nGenBank Accession Number: BC012043\nGene Symbol: HSD17B12\nGene ID (NCBI): 51144\nRRID: AB_3669997\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity Purification\nUNIPROT ID: Q53GQ0\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887671627945,"sku":"31463-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-31463-1-ap","provider":"Iright","version":"1.0","type":"link"}