{"product_id":"proteintech-51009-2-ap","title":"Proteintech, 51009-2-AP, BRD7 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BRD7 (51009-2-AP) by Proteintech is a Polyclonal antibody targeting BRD7 in WB, IHC, ELISA applications with reactivity to human,  mouse,   rat samples\n51009-2-AP targets BRD7 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human,  mouse,   rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, mouse cerebellum tissue,  mouse lung tissue,  L02 cells,  rat brain tissue,  SH-SY5Y cells,  HeLa cells,  Jurkat cells,  MDA-MB-231 cells,  PC-3 cells,  SH-5Y5Y cells\nPositive IHC detected in: human colon cancer tissue,  human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi\/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3911 Product name: Recombinant human BRD7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC050728 Sequence: MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEALNQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIYNKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSGDAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTTLGL Predict reactive species\nFull Name: bromodomain containing 7\nCalculated Molecular Weight: 651 aa, 74 kDa\nObserved Molecular Weight: 70-74 kDa\nGenBank Accession Number: BC050728\nGene Symbol: BRD7\nGene ID (NCBI): 29117\nRRID: AB_2259226\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NPI1\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868857487529,"sku":"51009-2-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-51009-2-ap","provider":"Iright","version":"1.0","type":"link"}