{"product_id":"proteintech-51068-1-ap","title":"Proteintech, 51068-1-AP, ERK1\/2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ERK1\/2 (51068-1-AP) by Proteintech is a Polyclonal antibody targeting ERK1\/2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human,  mouse,  rat samples\n51068-1-AP targets ERK1\/2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human,  mouse,  rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, mouse brain tissue,  Jurkat cells,  NIH\/3T3 cells,  mouse brain cells,  rat brain cells,  rat skin tissue\nPositive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:100-1:400\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1\/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, duck\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: mitogen-activated protein kinase 1\nCalculated Molecular Weight: 360 aa, 41 kDa\nObserved Molecular Weight: 38-44 kDa\nGenBank Accession Number: BC017832\nGene Symbol: ERK2\nGene ID (NCBI): 5594\nRRID: AB_2250380\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P28482\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868841037993,"sku":"51068-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-51068-1-ap","provider":"Iright","version":"1.0","type":"link"}