{"product_id":"proteintech-55028-1-ap","title":"Proteintech, 55028-1-AP, PFKM Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PFKM (55028-1-AP) by Proteintech is a Polyclonal antibody targeting PFKM in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n55028-1-AP targets PFKM in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  HepG2 cells,  human brain tissue,  human skeletal muscle tissue,  L02 cells,  mouse heart tissue,  rat heart tissue\nPositive IP detected in: mouse liver tissue\nPositive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue,  mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa. The antibody is specific to PFKM.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, chicken, bovine, sheep\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: phosphofructokinase, muscle\nCalculated Molecular Weight: 85 kDa\nObserved Molecular Weight: 75-85 kDa\nGenBank Accession Number: NM_000289\nGene Symbol: PFKM\nGene ID (NCBI): 5213\nRRID: AB_10858390\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P08237\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868832059561,"sku":"55028-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-55028-1-ap","provider":"Iright","version":"1.0","type":"link"}