{"product_id":"proteintech-55114-1-ap","title":"Proteintech, 55114-1-AP, NOTCH3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NOTCH3 (55114-1-AP) by Proteintech is a Polyclonal antibody targeting NOTCH3 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n55114-1-AP targets NOTCH3 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A2780 cells, Caco-2 cells,  U2OS cells,  COLO 320 cells,  HeLa cells,  K-562 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human lung cancer tissue, human breast cancer tissue,  human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: Notch homolog 3 (Drosophila)\nCalculated Molecular Weight: 244 kDa\nObserved Molecular Weight: 250-260 kDa, 100 kDa\nGenBank Accession Number: NM_000435\nGene Symbol: NOTCH3\nGene ID (NCBI): 4854\nRRID: AB_10858393\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UM47\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868837105833,"sku":"55114-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-55114-1-ap","provider":"Iright","version":"1.0","type":"link"}