{"product_id":"proteintech-55187-1-ap","title":"Proteintech, 55187-1-AP, ATP1A1-Specific Polyclonal antibody","description":"Size: 150ul\nThe ATP1A1-Specific (55187-1-AP) by Proteintech is a Polyclonal antibody targeting ATP1A1-Specific in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples\n55187-1-AP targets ATP1A1-Specific in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: 37°C incubated mouse brain tissue, HEK-293 cells,  mouse brain tissue,  mouse heart tissue,  Neuro-2a cells,  MCF-7 cells\nPositive IHC detected in: human ovary cancer tissue, human colon tissue,  human hepatocellular ca,  human liver cancer tissue,  mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells, Caco-2 cells\nPositive FC (Intra) detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nATP1A1 is the catalytic component of Na+\/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ATPase, Na+\/K+ transporting, alpha 1 polypeptide\nCalculated Molecular Weight: 113 kDa\nObserved Molecular Weight: 100-110 kDa\nGenBank Accession Number: NM_000701\nGene Symbol: ATP1A1\nGene ID (NCBI): 476\nENSEMBL Gene ID: ENSG00000163399\nRRID: AB_10859261\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P05023\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868867285161,"sku":"55187-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-55187-1-ap","provider":"Iright","version":"1.0","type":"link"}