{"product_id":"proteintech-55197-1-ap","title":"Proteintech, 55197-1-AP, PAPD5 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PAPD5 (55197-1-AP) by Proteintech is a Polyclonal antibody targeting PAPD5 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples\n55197-1-AP targets PAPD5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells\nPositive IP detected in: Jurkat cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPAPD5, also named as Non-canonical poly(A) RNA polymerase PAPD5, is a 572 amino acid protein, which contains 1 PAP-associated domain and belongs to the DNA polymerase type-B-like family. PAPD5 is predominantly expressed in the cytoplasm. PAPD5 as catalytic subunit of a TRAMP-like complex that has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. PAPD5 plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. PAPD5 also may play a role in sister chromatid cohesion. The calculated molecular weight of PAPD5 is a 63 kDa, but the post-modified protein is about 70-80 kDa. PAPD5 exists some isoform with the molecular weight about 50 kDa and 70-80 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: PAP associated domain containing 5\nCalculated Molecular Weight: 64 kDa\nObserved Molecular Weight: 63-80 kDa\nGenBank Accession Number: NM_001040285\nGene Symbol: PAPD5\nGene ID (NCBI): 64282\nRRID: AB_2881287\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8NDF8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869480079529,"sku":"55197-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-55197-1-ap","provider":"Iright","version":"1.0","type":"link"}