{"product_id":"proteintech-55213-1-ap","title":"Proteintech, 55213-1-AP, ARHGEF4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ARHGEF4 (55213-1-AP) by Proteintech is a Polyclonal antibody targeting ARHGEF4 in WB, IP, IHC, ELISA applications with reactivity to human,   mouse samples\n55213-1-AP targets ARHGEF4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human,   mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue,  multi-cells\/tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: Rho guanine nucleotide exchange factor (GEF) 4\nCalculated Molecular Weight: 79 kDa\nObserved Molecular Weight: 72 kDa\nGenBank Accession Number: NM_015320\nGene Symbol: ARHGEF4\nGene ID (NCBI): 50649\nRRID: AB_2721023\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NR80\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868947206313,"sku":"55213-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-55213-1-ap","provider":"Iright","version":"1.0","type":"link"}