{"product_id":"proteintech-55367-1-ap","title":"Proteintech, 55367-1-AP, EVC2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EVC2 (55367-1-AP) by Proteintech is a Polyclonal antibody targeting EVC2 in WB, IHC, ELISA applications with reactivity to Mouse, Human samples\n55367-1-AP targets EVC2 in WB, IHC, ELISA applications and shows reactivity with Mouse, Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse lung tissue\nPositive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEVC2 (also known as LIMBIN) is close proximity in divergent orientation to EVC and mutations in both human genes lead to the chondrodysplasia Ellis-van Creveld (EvC) syndrome. Typical symptoms of EvC include shorter limbs and ribs, postaxial polydactyly, dysplastic nails, and cardiovascular defects (PMID: 28081373). Additionally, EvC syndrome has been characterized as a ciliopathy because the EVC and EVC2 proteins are intracellularly localized at the bottom of the primary cilium, where they form a heterotrimeric protein complex with SMO and transduce Hedgehog(Hh) signaling (PMID: 30410447). EVC2 is expressed in the heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. EVC2 has 3 isoforms with the molecular mass of 140-148 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Mouse, Human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: Ellis van Creveld syndrome 2\nCalculated Molecular Weight: 148 kDa\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: NM_147127\nGene Symbol: EVC2\nGene ID (NCBI): 132884\nRRID: AB_3086445\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q86UK5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887628603561,"sku":"55367-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-55367-1-ap","provider":"Iright","version":"1.0","type":"link"}