{"product_id":"proteintech-55457-1-ap","title":"Proteintech, 55457-1-AP, UBE2E1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe UBE2E1 (55457-1-AP) by Proteintech is a Polyclonal antibody targeting UBE2E1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n55457-1-AP targets UBE2E1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2400\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nUBE2E1 is a class III E2 ubiquitin conjugating enzyme that belongs to the UBE2E family of E2s, comprising UBE2E1\/UBCH6, UBE2E2\/UBCH8, and UBE2E3\/UBCH9. UBE2E family members share a highly conserved UBC domain but are distinguished from one another by their unique N-terminal extensions. UbE2E1 contains a 50-residue unstructured N-terminal extension rich in serine and lysine residues, which has been shown to interact with USP7 and is essential for USP7-mediated UbE2E1 stabilization. In addition to its ubiquitin conjugating activity, UBE2E1 can also act as an ISG15 conjugating enzyme in vitro, although this activity has not been shown to occur in vivo. UBE2E1 can be covalently modified with either ISG15 or ubiquitin, both of which interfere with the ubiquitin conjugating activity of UBE2E1. UBE2E1 has also been reported to monoubiquitinate histone H2A at K119 in concert with the PRC1 E3 ligase complex. Full-length UBE2E1 is reported to be a dimer, with dimerization mediated by interaction of residues 21-27 from one molecule with the core domain of another molecule, as well as by contacts between a1 of the two interacting molecules. (PMID: 28073915, PMID: 30282802, PMID: 23871895)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ubiquitin-conjugating enzyme E2E 1 (UBC4\/5 homolog, yeast)\nCalculated Molecular Weight: 21 kDa\nObserved Molecular Weight: 21-27 kDa\nGenBank Accession Number: NM_003341\nGene Symbol: UBE2E1\nGene ID (NCBI): 7324\nRRID: AB_2918357\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P51965\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887913226409,"sku":"55457-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-55457-1-ap","provider":"Iright","version":"1.0","type":"link"}