{"product_id":"proteintech-60255-1-ig","title":"Proteintech, 60255-1-Ig, SMN-Exon7 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe SMN-Exon7 (60255-1-Ig) by Proteintech is a Monoclonal antibody targeting SMN-Exon7 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n60255-1-Ig targets SMN-Exon7 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells,  HeLa cells,  HepG2 cells\nPositive IHC detected in: mouse heart tissue, mouse brain tissue,  mouse pancreas tissue,  rat brain tissue,  rat heart tissue,  rat pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSpinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag16615 Product name: Recombinant human SMN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 275-294 aa of BC062723 Sequence: GYYMGFRQNQKEGRCSHSLN Predict reactive species\nFull Name: survival of motor neuron 1, telomeric\nCalculated Molecular Weight: 294 aa, 32 kDa\nObserved Molecular Weight: 40 kDa\nGenBank Accession Number: BC062723\nGene Symbol: SMN\nGene ID (NCBI): 6606\nRRID: AB_2881376\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q16637\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888050065577,"sku":"60255-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-60255-1-ig","provider":"Iright","version":"1.0","type":"link"}