{"product_id":"proteintech-60555-1-ig","title":"Proteintech, 60555-1-Ig, Cleaved PARP1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Cleaved PARP1 (60555-1-Ig) by Proteintech is a Monoclonal antibody targeting Cleaved PARP1 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n60555-1-Ig targets Cleaved PARP1 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A2780 cells, HSC-T6 cells,  mouse splenocytes,  Staurosporine treated A2780 cells,  Staurosporine treated HSC-T6 cells,  Staurosporine treated mouse splenocytes\nPositive IHC detected in: Jurkat cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: 1 μM  Staurosporine (3 hours) treated HSC-T6 cells, 1 μM  Staurosporine (3 hours) treated HeLa cells\nPositive FC (Intra) detected in: 1 μM  Staurosporine (3 hours) treated HSC-T6 cells, 1 μM  Staurosporine (3 hours) treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: poly (ADP-ribose) polymerase 1\nCalculated Molecular Weight: 1014 aa, 113 kDa\nObserved Molecular Weight: 89 kDa\nGenBank Accession Number: BC037545\nGene Symbol: PARP1\nGene ID (NCBI): 142\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P09874\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887966212265,"sku":"60555-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-60555-1-ig","provider":"Iright","version":"1.0","type":"link"}