{"product_id":"proteintech-65122-1-ig","title":"Proteintech, 65122-1-Ig, Anti-Mouse MHC Class II (I-A\/I-E) (M5\/114.15.2)","description":"Size: 100ug \/ 500ug\nThe MHC Class II (I-A\/I-E) (65122-1-Ig) by Proteintech is a Monoclonal antibody targeting MHC Class II (I-A\/I-E) in FC applications with reactivity to Mouse samples\n65122-1-Ig targets MHC Class II (I-A\/I-E) in IF, FC applications and shows reactivity with Mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive FC detected in: mouse splenocytes\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nFlow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMajor histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5\/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5\/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Mouse\nCited Reactivity: mouse\nHost \/ Isotype: Rat \/ IgG2b, kappa\nClass: Monoclonal\nType: Antibody\nImmunogen: Activated C57BL\/6 mouse spleen cells Predict reactive species\nFull Name: histocompatibility 2, class II antigen A, alpha\nGenBank Accession Number: BC031711\nGene Symbol: MHC Class II\nGene ID (NCBI): 14960\nRRID: AB_2918416\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Affinity purification\nUNIPROT ID: P14434\nStorage Buffer: PBS with 0.09% sodium azide, pH 7.3.\nStorage Conditions: Store at 2-8°C. Stable for one year after shipment.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888025030825,"sku":"65122-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-65122-1-ig","provider":"Iright","version":"1.0","type":"link"}