{"product_id":"proteintech-65139-1-ig","title":"Proteintech, 65139-1-Ig, Anti-Mouse CD45R (B220) (RA3-6B2)","description":"Size: 500ug\nThe CD45R (B220) (65139-1-Ig) by Proteintech is a Monoclonal antibody targeting CD45R (B220) in IHC, IF\/ICC, IF-P, FC applications with reactivity to mouse samples\n65139-1-Ig targets CD45R (B220) in IHC, IF\/ICC, IF-P, FC applications and shows reactivity with mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse spleen tissue\nPositive IF\/ICC detected in: mouse splenocytes\nPositive FC detected in: mouse splenocytes\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: mouse\nCited Reactivity: mouse\nHost \/ Isotype: Rat \/ IgG2a, kappa\nClass: Monoclonal\nType: Antibody\nImmunogen: Abelson murine leukemia virus-induced pre-B tumor cells Predict reactive species\nFull Name: protein tyrosine phosphatase, receptor type, C\nGenBank Accession Number: BC028512\nGene Symbol: CD45\nGene ID (NCBI): 19264\nRRID: AB_2918426\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Affinity purification\nUNIPROT ID: P06800\nStorage Buffer: PBS with 0.09% sodium azide, pH 7.3.\nStorage Conditions: Store at 2-8°C. Stable for one year after shipment.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888054882473,"sku":"65139-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-65139-1-ig","provider":"Iright","version":"1.0","type":"link"}